| #1 Red West |
| 5001 |
North Grand Mall |
| 5010 |
Duff Avenue at Kellogg Avenue Eastbound |
| 5021 |
Duff Avenue at Northwood Drive Southbound |
| 5031 |
Duff Avenue at 24th Street Southbound |
| 5041 |
Duff Avenue at 22nd Street Southbound |
| 5051 |
Duff Avenue at 20th Street Westbound |
| 5061 |
Duff Avenue at O'Neil Drive Westbound |
| 5071 |
Duff Avenue at 16th Street Westbound |
| 5081 |
Duff Avenue at 14th Street Westbound |
| 5091 |
Duff Avenue at McFarland Clinic Southbound |
| 5101 |
Mary Greely Hospital Southbound |
| 5111 |
Duff Avenue at 9th Street Westbound |
| 5121 |
Duff Avenue at 8th Street Westbound |
| 5131 |
6th Street at Duff Avenue Westbound |
| 5201 |
Ames Public Library Southbound |
| 5211 |
5th Street at Kellogg Avenue Westbound |
| 5501 |
City Hall Westbound |
| 5510 |
Allan Drive at Grand Avenue |
| 5521 |
Iowa Department of Transportation Westbound |
| 5531 |
Lincoln Way at Maple Avenue Westbound |
| 5541 |
Lincoln Way at Hazel Avenue Westbound |
| 5551 |
Lincoln Way at Russell Avenue Westbound |
| 5561 |
Lincoln Way at Hilton Coliseum Westbound |
| 2001 |
Lincoln Way at Beach Avenue Westbound |
| 2011 |
Lincoln Way at Union Drive Westbound |
| 2021 |
Lincoln Way at Lynn Avenue Westbound |
| 2040 |
Lake Laverne West |
| 2051 |
Student Services |
| 2061 |
Beyer Hall |
| 2071 |
West Street at Hyland Avenue Westbound |
| 3591 |
Hyland Avenue at Lincoln Way Southbound |
| 3581 |
Lincoln Way at State Avenue Westbound |
| 3571 |
Lincoln Way at Wilmoth Avenue Westbound |
| 3561 |
Lincoln Way at Franklin Avenue Westbound |
| 3551 |
Lincoln Way at Marshall Avenue Westbound |
| 3541 |
Lincoln Way at Hickory Drive Westbound |
| 3521 |
South Dakota Avenue at Lincoln Swing Southbound |
| 3511 |
South Dakota Avenue at Todd Drive Southbound |
| 3501 |
South Dakota Avenue at Clemens Boulevard Southbound |
| 3494 |
South Dakota Avenue at Steinbeck Street Southbound |
| 3300 |
Mortensen Road at Coconino Road Eastbound |
| 3310 |
Mortensen Road at Dotson Drive Eastbound |
| 3333 |
Ames Middle School |
